Interaction Mechanism in Dye-Ligand Affinity Chromatography
نویسندگان
چکیده
منابع مشابه
Triazine-dye affinity; chromatography.
functional resemblance of column groups to carbamoyl phosphate. The enzyme binds its two substrates, carbamoyl phosphate and L-aspartate, in an obligatory order, with carbamoyl phosphate leading (Grayson et al., 1979). If column groups are sufficiently similar to carbamoyl phosphate, the enzyme should be adsorbed more tightly in the presence of L-aspartate, owing to the ‘locking-on’ effect (O’C...
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Pyruvate carboxylase (PC) was purified to homogeneity from an overexpressing strain of the purple photosynthetic bacterium Rhodobacter capsulatus using a rapid dye-ligand affinity chromatography procedure, in which dye-bound enzyme was specifically eluted with a low concentration of acetyl-CoA, an allosteric activator of the enzyme. The enzyme purified by this method was obtained in 75% yield w...
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ژورنال
عنوان ژورنال: Chemie Ingenieur Technik
سال: 2014
ISSN: 0009-286X
DOI: 10.1002/cite.201450026